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KMID : 0903620000410030297
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Journal of the Korean Society for Horticultural Science
2000 Volume.41 No. 3 p.297 ~ p.300
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Effects of Growth Regulators and Culture Method on Shoot Formation from Microscale in Lilium Oriental Hybrid ´ Casa Blanca ´
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이현숙/Lee, Hyun Suk
김규원/한윤열/심용구/우진하/최경배/최정두/Kim, Kiu Weon/Han, Youn Yol/Sim, Yong Gu/Woo, Jin Ha/Choi, Kyeong Bae/Choi, Jeong Doo
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Abstract
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This study was performed to investigate the effects of growth regulators and culture methods on shoot formation from microscale in Lilium Oriental Hybrid ´Casa Blanca´. To determine the optimum concentration of NAA and TDZ for shoots formation the continuous linear gradient consisted of 300 mL of 10 ㎎·L^(-1) NAA or TDZ and 300 mL distilled water. Optimum concentration was determined to be 5.0 ㎎·L^(-1) NAA and 3.16 ㎎·L^(-1) TDZ in the subdivisional system. 0.1 ㎎·L^(-1) NAA combined with 1.0 ㎎·L^(-1) BA was remarkably effective in formation and growth of the shoot. Number of shoots was higher on MS medium containing 0.5 ㎎·L^(-1) NAA and 0.1 to 0.5 ㎎·L^(-1) TDZ than other treatments. The number of shoots increased when grown under 16-h photoperiod. In pre-light culture and post-dark culture the number of shoots increased as the light period increased. Conversely, in pre-dark and post-light dark culture, the number of shoots increased as the dark period increased. Shoot formation and growth were higher in liquid culture than in solid and membrane raft and float system (MRFS) culture.
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KEYWORD
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